Aim: The aim of this study was to evaluate the relationship between p16 / Ki-67 dual staining used for the definition of precancerous cervical lesions with histological results and HPV positivity. Materials and Method: This study is a cross-sectional study of 468 patients who were followed up in our center with the diagnosis of cervical intraepithelial neoplasia between 2016 and 2019 using the cytology, colposcopic biopsy and conization results, HPV test and p16 / Ki-67 dual staining results. SPSS 22 program was used in the analysis of the data. In the analysis of qualitative data, chi-square test and binary logistic regression analysis were used. The compatibility of both models with bilateral logistic regression test was good (omnibus test < 0.001). The correct estimate percentage of the model is 71.4% and 80.3%. p <0.05 is considered important. Results: In the binary logistic regression test established between HPV types and p16 / Ki-67 dual staining positivity, other high risk HPV types, HPV 16, 18 and 16-18, increased p16 / Ki-67 positivity ratio in this order. In the binary logistic regression test established between abnormal cytology and p16 / Ki-67 dual staining positivity in the colposcopy results, there was p16 / Ki-67 positivity increasing in proportion with the degree of HGSIL lesions. Conclusion: This study created with cytology / colposcopy and LEEP conization protocol shows that; in effective screening for early diagnosis and treatment in cervical cancer, p16 / Ki-67 biomarkers can be used effectively.