Objective: This study aims to investigate the expression of the HIWI gene and explore its relationship with endometrial carcinoma. Materials and Methods: This study included 72 endometrial carcinoma (EC) cases. The study also analyzed 68 cases of endometrial benign disease and 78 cases of normal endometrium. Immunohistochemistry and western blot were used to detect the expression of HIWI in different tissues. Results: The expression level of HIWI was significantly higher in endometrial carcinoma than in benign endometrium and normal endometrium (P < 0.05). In addition, HIWI expression was significantly correlated with tumor myometrial invasion depth, cervical invasion, lymph vascular invasion, lymph node metastasis and clinical stage (P < 0.05), but was not correlated with the age or menstrual period of patients (P > 0.05). Conclusion: HIWI is correlated to the occurrence and development of endometrial carcinoma, and may be one of the factors that affect the prognosis of endometrial carcinoma.