Human microbiome study has become pivotal during the last years; eubiosis, pathobiosis, and balanced ecosystem are now leading terms in comprehension of Health and Disease in humans. Vaginal ecosystem is a balance system in which microbiome is in constant dynamic profile in relation to the symbiotic equilibrium from different species and cells (human cells, lactobacilli and other bacteria, virus or fungi. Previous experiences demonstrated how it is possible to restore human vaginal ecosystem by using a long-time course of a selected population of vaginal symbiotic containing lactobacillus rhamnosus BMX 54 plus lactose. Taking into account the leading role of lactobaciili in human vaginal microbiota and the pathobiosis pathway from eubiosis to pathology until cancer (eubiosis derangement versus a more aggressive and dangerous pathobiosis), the authors hypothesized that exogenously manipulation of vaginal microbiome by a selected symbiotic could stop the pathobiosis pathway leading to a new health system. Surprisingly a recent published clinical trial showed a consistent percentage of clearance of PAP-smear abnormalities and HPV-DNA clearance obtained in women affected by concomitant vaginitis/bacterial vaginosis and HPV infections treated for long-term course with vaginal tabs of lactobacillus rhamnosus BMX 54 plus lactose. Since exogenous vaginal manipulation of microbiome could be a new and promising tool for eubiosis restoration in vaginal ecosystem, and since “pathobiosis pathway” could be interrupted by long-term use of vaginal probiotics, it seems possible to confirm the hypothesis that eubiosis replacement in vaginal microbiome could lead to counter infections such as HPV and its detrimental-related pathologies in women. Vaginal probiotics application could represent a safe, less expensive, and effective treatment to control HPV infections.