Purpose: The objective of this study was to verify the relationship between the expression of VDP1 and Jun activation domain-binding protein 1 (Jab1) and cyclin-dependent kinase inhibitor 1B (CDKN1B: p27kip1) in ovarian cancer cell strains. Materials and Methods: The ovarian cancer cell lines SK-OV-3, OVCAR-3 were plated and cultivated for 24 hours in a 37°C carbon dioxide incubator. Thereafter, vitamin D was added at various concentrations, and the cells were cultivated for an additional 24 hours. Using the 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide (MTT) assay, cell growth was checked, and changes in the protein level were observed using Western blotting, immunofluorescence and immunocytochemistry. Results: The authors found that the viability of the ovarian cancer cells was decreased by vitamin D, dose dependently. The results of Western blotting, immunofluorescence, and immunocytochemistry revealed that p27kip1 and VDUP1 increased, while Jab1 decreased, dose dependently. Conclusion: These findings show the relationship between p27kip1, Jab1 and VDUP1 following vitamin D treatment in ovarian cancer, indicating a correlation with cancer cell proliferation.