Adenoid cystic carcinoma (ACC) is an uncommon histological type of the already rare carcinoma of Bartholin’s gland. The authors present a case of a 45-year-old woman who was admitted with a two-month history of vulvar pain. Physical examination revealed a 2×2- cm fixed mass on the right vaginal wall, originating from the area of Bartholin’s gland. As the mass was located in deep tissue, a biopsy could not be performed. Therefore, local excision of the mass was performed under general anesthesia. Pathology examination revealed an ACC of Bartholin’s gland. Because of malignancy diagnosis, a more radical surgery was mandatory: a posterior hemivulvectomy, obtaining clear margins, and bilateral inguinofemoral node dissection were performed. In this type of cancer, local recurrences are common and often precede distant metastases.