Aim: This study aimed to investigate the mechanism by which progesterone receptors (PRs) mediate in metastatic spread of endometrial cancer (EC). Materials and Methods: The expressions of human progesterone receptor isoforms A and B (hPRA and hPRB) in Ishikawa endometrial cancer cells transfected were examined by Western blot. Matrigel invasion assay was performed to illustrate the roles of hPRA and hPRB in EC metastasis.The underlying mechanism of PR regulating invasion was assessed by Western blot and RT-PCR. Results: In this study ,the authors found a significant increased expression of hPRA and hPRB in transfected cells, respectively, whereas hPRB exhibited a slightly decreased expression upon hPRA stimulation. Matrigel invasion assay demonstrated that hPRB decreased cell invasion, and this process could be largely restored by hPRA treatment. Further study of potential mechanism indicated that hPRB upregulated the expression of SULT1E1, which was connected with endometrial oncogenesis, and the suppression of hPRB via hPRA, exerted an inhibitory effect on the expression of SULT1E1. Conclusions: The above data showed that hPRB promoted the expression of SLUT1E1 to inhibite the invasion of EC cells. In contrast, hPRA played an opposite role in attenuating the effects of hPRB on the invasion of Ishikawa cells and the expression of SLUT1E1.