Objective: The aims of this study were to explore the correlation between MiR-34s and cervical lesions. Materials and Methods: A casecontrol study was conducted. From 2014 to 2015, 120 cases were included and divided into four groups depending on cervical biopsy, including group 0 (cervicitis or normal cervix), group 1 (CINI), group 2 (CINII-III), and group C (cervical cancer); each group included 30 cases. Then the relationship between miRNA-34 family (including miRNA-34a, miRNA-34b, miRNA-34c) and clinical pathological features was analysed based on detection of miRNA-34 by real-time polymerase chain reaction (RT-PCR). Results: The expression level of miRNA-34a in group 0 was 0.19 ± 0.1073, in group 1 it was 0.1507 ±0. 2124, in group 2 it was 0.0766 ± 0.0948 (vs. group 0: p < 0.05), and in group C it was 0.0501 ± 0.0271 (vs. Group 0: p < 0.05). The correlation analysis showed that the expression of miRNA-34a decreased with the aggravating of the cervical lesion (r = -0.4782, p < 0.05), while there was no difference of miRNA-34b (F = 0.5835, p = 0.6282) or miRNA-34c (F = 0.1167, p = 0.9500) in each group. Conclusion: The decrease of miRNA-34a is related with cervical malignant lesion, which may be involved in the pathogenesis by regulating target genes, that may be a new molecular marker for diagnosis and prognosis of cervical cancer.