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European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.
Original Research
MiR-182-5p promotes proliferation and invasion by regulating Smad4 in vulvar squamous cell cancer
Xiuhua Yang1, Xin Wu2, *
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1
Department of Obstetrics, The First Hospital of China Medical University, Shenyang, Liaoning, China
2
Department of Gynaecology, The First Hospital of China Medical University, Shenyang, Liaoning, China
Eur. J. Gynaecol. Oncol. 2018, 39(4), 604–608;
https://doi.org/10.12892/ejgo4177.2018
Published: 10 August 2018
Abstract
The purpose of this study was to investigate the potential mechanism of miR-182-5p and Smad4 in vulvar squamous cell carcinoma (VSCC). A431 cells were transfected with miR-182-5p mimics, a small interfering RNA targeting Smad4 (siR-Smad4) and their negative controls. The authors performed gene functional experiments in A431 cells. The protein levels of MMP-2, MMP-9, E-cadherin, N-cadherin, and vimentin were detected after transfection miR-182-5p mimics. The expression levels of Smad4, p21, plasminogen activator inhibitor-1 (PAI-1), Bax, and Bim were detected after transfection miR-182-5p mimics. In A431 cells, up-expression of miR-182- 5p advanced the cell proliferation, migration, invasion, G1-S phase transition, and down-regulated Smad4; miR-182-5p also increased the expressions of MMP-2 and MMP-9 and influenced alterations in epithelial-mesenchymal transition (EMT) and suppressed downstream target genes of Smad4. The down-regulation of Smad4 by siRNA simulated the roles of miR-182-5p. The authors conclude that miR-182-5p plays an oncogenic role by its target gene Smad4 in VSCC.
Keywords
Vulvar squamous cell carcinoma
miR-182-5p
Smad4
Epithelial-mesenchymal transition