Background: Mature cystic teratomas are the most frequent ovarian tumors; however, teratomas of extragonadal origin are extremely rare. The authors describe herein the first case simultaneously demonstrating normal ovaries and a mature cystic teratoma in the vesicovaginal space on laparoscopy. Case Report: A 23-year-old Korean nulliparous woman, with no disease history, was referred to the present outpatient clinic for an asymptomatic pelvic mass detected on routine checkup following a traffic accident. She underwent laparoscopy under a preoperative diagnosis of subserosal myoma in the left anterior wall of the uterus. A benign cystic tumor was found in the vesicovaginal space and was completely treated via laparoscopic resection. Conclusion: A mature cystic teratoma in the vesicovaginal space is extremely rare, and proper preoperative diagnosis of this lesion is difficult because of non-specific signs and symptoms. Although uncommon, teratomas should be considered in the differential diagnosis of extragonadal pelvic masses, especially in young adults.