IMR Press / EJGO / Volume 39 / Issue 1 / DOI: 10.12892/ejgo3843.2018

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with S.O.G.

Original Research
Screening crucial genes involved in triple-negative breast cancer through bioinformatics analysis of microarray data
Show Less
1 Department of Hematology and Oncology, China-Japan Union Hospital of Jilin University, Changchun, China
2 Department of Nephrology, The First Hospital of Jilin University, Changchun, China
3 Department of Rheumatology and Immunology, China-Japan Union Hospital of Jilin University, Changchun, China
Eur. J. Gynaecol. Oncol. 2018, 39(1), 101–107;
Published: 10 February 2018

Purpose: Triple-negative breast cancer (TNBC) is breast cancer with estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2) negative. TNBC patients have a high risk of distant recurrence and death. This study aimed to investigate the mechanisms of TNBC. Materials and Methods: GSE38959 downloaded from Gene Expression Omnibus database included 30 TNBC cells and 13 normal mammary gland ductal cells. The differentially expressed genes (DEGs) were identified using limma package in a bioconductor and then annotated. Using the Database for Annotation, Visualization and Integrated Discovery (DAVID) tool, their functions were predicted through enrichment analysis. After the protein-protein interaction (PPI) interactions were searched using STRING database, PPI network and modules were constructed by Cytoscape software. Results: A total of 2,039 DEGs were identified in TNBC cells. Among the upregulated genes, there were 36 TFs and 78 TAGs. Among the downregulated genes, a total of 46 TFs and 89 TAGs were annotated. Three significant modules were identified from the PPI network for the DEGs. Enrichment analysis showed that CCNB1, CDK1, PLK1, BUB1, and BUB1B were enriched in cell cycle, meanwhile, PSMD4 was enriched in the proteasome pathway. Genes might also function in TNBC through interacting with others (e.g. CDK1-CCNB1, PSMD14-UCHL5, BUB1-PLK1, and BUB1-BUB1B). Conclusion: In conclusion, CDK1 and CCNB1 were the key genes involved in the proliferation and apoptosis of TNBC cells. BUB1, BUBR1, and PLK1 might play crucial roles in the chromosomal instability in TNBC development. The interaction between UCHL5 and PSMD14 might be the pivotal mechanism in the degradation of ER in TNBC.
Triple-negative breast cancer
Differentially expressed genes
Enrichment analysis
Protein-protein interaction network
Back to top