Objective: MicroRNA-452 (miR-452) was previously reported to be dysregulated in several types of human cancers and involved in tumor progression. However, its role in cervical cancer is still unclear. The aim of this study was to investigate the clinical significance and prognostic value of miR-452 expression in human cervical cancer. Materials and Methods: The expressions of miR-452 was detected in 137 pairs of cervical cancer specimens and adjacent non-cancerous tissues using qRT-PCR assay. Then, the association of miR-452 levels with clinicopathological features and prognosis was evaluated. Results: MiR-452 expression was significantly downregulated in cervical cancer tissues than those corresponding non-cancerous tissues (p < 0.001). Decreased miR-452 expression was linked to lymph node metastasis, vascular invasion, poor tumor differentiation, advanced FIGO Stage, and short overall survival. Multivariate Cox regression analysis confirmed that low level of miR-452 expression predicted poor prognosis of patients with cervical cancer independently. Conclusions: The present results suggest that miR-452 downregulation may be involved in cervical cancer formation and progression, and that miR-452 would serve as a novel prognostic biomarker for patients with this disease.