Purpose of investigation: Long non-coding RNA (lncRNA) urothelial carcinoma-associated 1 (UCA1) has been found to be upregulated in number of cancers; however, the contributions of UCA1 to high grade serous ovarian carcinoma (HGSOC) remain largely unknown. The aim of the current study was to investigate the clinical significance and biological function of UCA1 in HGSOC. Materials and Methods: Detectable or altered level of UCA1 was evaluated in HGSOC tissue and cell lines using quantitative real-time polymerase chain reaction. The potential relationship between UCA1 levels in tumour tissues and the clinico-pathological features of HGSOC was further established by using cell motility and invasion assay, apoptosis analysis, and cell cycle analysis. Results: The present results demonstrated that UCA1 levels are markedly increased in HGSOC tissues and correlated with larger tumour size, less differentiated histology, prognosis, and greater tumour depth. Conclusion: Data from this study suggests an important role for UCA1 in the molecular etiology, diagnosis, and progression of HGSOC.