IMR Press / EJGO / Volume 38 / Issue 3 / DOI: 10.12892/ejgo3692.2017

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with S.O.G.

Open Access Original Research
HPV-associated cervical cancer cells targeted by triblock copolymer gold nanoparticle curcumin combination
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1 Gynecology and Obstetrics Department, Loma Linda University School of Medicine, Loma Linda, CA, USA
2 Department of Basic Sciences, Center for Health Disparities and Molecular Medicine, Loma Linda University School of Medicine, Loma Linda, CA, USA
Eur. J. Gynaecol. Oncol. 2017, 38(3), 413–417;
Published: 10 June 2017

Objective: Curcumin (diferuloylmethane) has promising anti-cervical cancer properties but requires a stabilizing complex such as the Pluronic triblock copolymer gold nanoparticle (GNP). The objectives were to study cytotoxicity of curcumin and to determine the effect of copolymer GNPs curcumin complex on cancer cell necrosis. Materials and Methods: The HeLa cells were maintained in Eagle Minimal Essential Medium, fetal bovine serum, and antibiotics, and passaged until 60 % confluency was reached. The cells were exposed to either: (1) control medium, (2) 50 μM curcumin, (3) 100 μM curcumin, (4) 50 μM curcumin with copolymer GNPs complex, or (5) 100 μM curcumin with copolymer GNPs complex. The treated cells were incubated at 37°C with 5% CO2 in air for 24 hours, and analyzed for viability, apoptosis or necrosis using the dual stains fluorescence procedure. Results: A dose-dependent increase in the HeLa necrosis was observed with increasing curcumin concentrations. Cytotoxic effect was decreased by five- to ten-fold when the curcumin was complexed with copolymer GNPs. There were more apoptotic HeLa cells at the higher concentration of curcumin but combination with copolymer GNPs resulted in decreased apoptosis. Cell viability was higher in curcumin with copolymer GNPs (74.4 ± 4.8 versus 2.3 ± 2.2 % live, mean ± SEM, with and without copolymer GNPs, respectively). Conclusion: Curcumin increased HeLa cancer cell necrosis but its cytotoxicity was decreased by copolymer GNPs. The results suggested that this specific copolymer GNP did not enhance the curcumin bioavailability in cultured cells possibly due to formation of copolymer GNP aggregates.
Human papillomavirus
Triblock copolymer
Gold nanoparticles
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