Objective: The aim of this study was to evaluate the clinical value of ultrasound-guided resection of non-palpable breast masses. Materials and Methods: One hundred thirty-seven cases of non palpable breast mass patients who underwent surgical treatment from June 2007 to June 2012 were enrolled in this study. All patients were found with breast masses in mammography or ultrasound, and underwent preoperative routine ultrasound-guided wire positioning. Results: One hundred fifty-eight lesions underwent ultrasound positioning accurate resection, of which 34 cases (21.5%) were malignant, including 26 cases (16.5%) of intraductal carcinoma, four cases (2.5%) of infiltrating ductal carcinoma, and three cases (1.9%) of invasive lobular carcinoma. Thirteen patients underwent modified radical mastectomy, and 21 cases (61.8%) underwent breast conservation operation. All patients were followed up for six to 53 months, only one patient had pulmonary metastasis after two years, and there was no local recurrence. Conclusion: Resection of non-palpable breast masses with preoperative ultrasound-guided positioning has advantages of accurate positioning and simple operation and is of great significance for early diagnosis and treatment of breast cancer.