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European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.
Hydroxycamptothecin shows antitumor efficacy on HeLa cells via autophagy activation mediated apoptosis in cervical cancer
Y.X. Cheng1, Q.F. Zhang1, F. Pan2, J.L. Huang1, B.L. Li1, M. Hu1, M.Q. Li1, Ch. Chen3,*
1 Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, China
2 Department of Orthopaedics, Renmin Hospital of Wuhan University, Wuhan, China
3 Department of Breast and Thyriod Surgery, Renmin Hospital of Wuhan University, Wuhan, China
Eur. J. Gynaecol. Oncol. 2016, 37(2), 238–243; https://doi.org/10.12892/ejgo2845.2016
Published: 10 April 2016
Objectives: To investigate the effect and the mechanism of anti-tumor agent hydroxycamptothecin (HCPT) on HeLa cells in cervical cancer. Materials and Methods: Autophagy and apoptosis were detected by western blotting and the transfection of GFP-LC3 shRNA as well as Hoechst staining. Results: The authors found that the expression of the regulators of Beclin-1, p62, and microtubule-associated protein 1 light chain 3 (LC3) upregulated and then triggered the occurrence of cell autophagy. On the other hand, HCPT could induce to the formation of autophagy and resulted in cell apoptosis after autophagy. Conclusion: HCPT can alter cell autophagy and then trigger cell apoptosis to achieve antitumor effects.