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European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.
Original Research
Metastasis gene expression analyses of choriocarcinoma and the effect of silencing metastasis-associated genes on metastatic ability of choriocarcinoma cells
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1
Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University, Changsha, Hunan
2
Department of Gynecological Oncology, Hunan Tumor Hospital
3
Shenzhen Maternity and Child Healthcare Hospital, Shenzhen, Guangzhou (People’s Republic of China)
Eur. J. Gynaecol. Oncol. 2011, 32(3), 264–268;
Published: 10 June 2011
Abstract
Objective: Obtaining choriocarcinoma metastasis-associated genes and identifying the role and mechanism of VEGF-B in the progression of human choriocarcinoma. Study Design: (1) cDNA microarray technique was used to compare the transcriptional profiles between highly metastatic JEG-3 cells and lowly metastatic JAR cells; (2) An inhibitory effect of VEGF-B shRNA was demonstrated by RT-PCR; (3) The effect of VEGF-B shRNA on invasion of JEG-3 cells in vitro was detected by Matrigel invasion assay. Results: (1) In upregulated genes, 51 genes were correlated with the cell metastasis ability, and FN, MMP-2, uPA, CAV-1 and VEGFB were the first five genes; (2) Afterwards transfected VEGF-B shRNA, VEGF-B mRNA expression decreased obviously; (3) VEGFB shRNA transfection significantly downregulated invasion level of JEG-3 cells in vitro (p < 0.05). Conclusion: VEGF-B plays an important role in the metastatic capability of human choriocarcinoma. Reducing the expression of VEGF-B can help weaken the invasion ability of human choriocarcinoma.
Keywords
cDNA microarray
RNAi
VEGF-B
Choriocarcinoma
Invasion
Metastasis