IMR Press / EJGO / Volume 30 / Issue 5 / pii/2009120

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with S.O.G.

Original Research

Assessment of proliferating activity in Paget’s disease of the nipple by double stain immunohistochemistry

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1 Departments of Gynecology, Erasme Hospital, Free University of Brussels (ULB), Brussels (Belgium)
2 Departments of Pathology, Erasme Hospital, Free University of Brussels (ULB), Brussels (Belgium)
Eur. J. Gynaecol. Oncol. 2009, 30(5), 500–502;
Published: 10 October 2009

Paget disease of the nipple is a rare disease characterized by the presence of malignant glandular cells within the squamous epithelium of the nipple. The most common hypothesis to explain the development of Paget’s disease is an intraepithelial epidermotropic migration of malignant epithelial cells originating from an underlying intraductal carcinoma. Although the immunohistochemical properties of Paget cells in the nipple have been extensively studied, their proliferating characteristics remain paradoxically poorly studied. In the present study we have investigated the proliferating activity of Paget cells in the nipple by using double stain immunohistochemistry with both Ki-67 (a protein which is expressed in all active parts of the cell cycle) and cytokeratin 7 (a highly sensitive marker of Paget cells). Ten cases of Paget’s disease and the associated intraductal carcinomas (n = 10) and/or invasive carcinomas (n = 4) were tested. The mean Ki-67 index was in Paget’s disease (26% ± 10), in intraductal carcinomas (23% ± 8) and/or in invasive carcinomas (20% ± 8) (p > 0.05). This is the first report to convincingly demonstrate by specific double stain immunohistochemistry that Paget’s disease and underlying intraductal carcinomas share a close proliferating activity.

Paget’s disease
Double stain immunohistochemistry
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