IMR Press / EJGO / Volume 29 / Issue 3 / pii/1630995583171-928224494

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with S.O.G.

Original Research
Discovery of altered protein profiles in epithelial ovarian carcinogenesis by SELDI mass spectrometry
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1 Department of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou (China)
2 Department of Oncology, Cancer Institute, Clinical Laboratory, Second Affiliated Hospital of Zhejiang University College of Medicine, Hangzhou (China)
Eur. J. Gynaecol. Oncol. 2008, 29(3), 233–238;
Published: 10 June 2008

Objective: Identification of proteomic alterations in epithelial ovarian tumorigenesis may facilitate the understanding of progression of this disease. Methods: Specific protein peak patterns were identified in 20 microdissected epithelial ovarian tumors (13 epithelial ovarian cancers (EOCs) and 7 low malignant potential (LMP) tumors), as well as in the matched normal cells. Protein profiles were generated by surface-enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) from all the different types of cells. Results: Among seven protein peaks from EOC cells, six were significantly increased while one was decreased compared with normal cells, and three peaks from LMP cells were markedly increased while one was decreased compared with normal cells. Conclusions: The combination of SELDI and laser capture microdissection (LCM) is effective in finding the key molecules in ovarian tumorigenesis. Further identification of these protein peaks is important and these malignant protein signatures lend themselves to identification of populations at high-risk for EOC and for monitoring response to EOC chemopreventive agents.
Epithelial ovarian cancer
Surface-enhanced laser desorption/ionization mass spectrometry (SELDI-MS)
Laser capture microdissection (LCM).
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