IMR Press / EJGO / Volume 25 / Issue 3 / pii/2004167

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Original Research

Establishment and characterization of two different types of new human endometrial adenocarcinoma cell lines

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1 Department of Clinical Cytology, Graduate School of Medical Sciences, Kanagawa (Japan)
2 Tissue Culture Center, School of Medicine, Kitasato University, Kanagawa (Japan)
3 Department of Obstetrics and Gynecology, School of Medicine, Kitasato University, Kanagawa (Japan)
4 Department of Pathology, School of Medicine, Kitasato University, Kanagawa (Japan)
Eur. J. Gynaecol. Oncol. 2004, 25(3), 299–304;
Published: 10 June 2004
Abstract

Objective: Endometrial carcinomas are grouped into two types of carcinogenic entities. These two different types of cell lines designated as HEC-251 and HEC-265 were established from human endometrial adenocarcinoma. We report their characteristics. Materials: HEC-251 was derived from moderately differentiated endometrioid adenocarcinoma and HEC-265 from well-differ­entiated adenocarcinoma. Results: These cell lines grow well and serial passages can be successively carried out more than 100 times. The monolayer cul­tured cells reveal neoplastic and pleomorphic features, and grow in multilayers. HEC-251 cells are immuno-cytochemically posi­tive for p53 and HEC-265 cells for PgR. Both cell lines are transplantable to nude mice and reflect the original histopathologic char­acteristics. Conclusion: The cell lines HEC-251 and HEC-265 will contribute to clarifying the characteristics of two different types of human endometrial carcinomas.

Keywords
Endometrial adenocarcinoma
Uterine neoplasm
Cell line
p53
PgR
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