IMR Press / EJGO / Volume 20 / Issue 4 / pii/1999170

European Journal of Gynaecological Oncology (EJGO) is published by IMR Press from Volume 40 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with S.O.G.

Original Research

Characterization of soluble factors from cultures of premalignant cervical epithelium

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1 Department of Immunology, University College Lcmdon Medical School, London, UK
2 Institute of Ophthalmology, UCL, London, UK
3 Women's Health Services, Whittington Hospital, London, UK
Eur. J. Gynaecol. Oncol. 1999, 20(4), 262–267;
Published: 10 August 1999

Purpose: Immune responses within the cervical microenvironment are likely to play an important role in the natural history of premalignant lesions but the pattern of this response and how it is regulated has not been documented in detail. Methods: Explants of premalignant cervical epithelium were cultured in vitro for 24 hours. The culture supernatants were assayed for the presence of IL-1α, IL-10, IL-12 and TNF-α by ELISA. Aliquots of each supernatant were also added to a CD3-dependent T cell proliferation assay. Results: The pattern of cytokines found in different samples was heterogeneous and no significant correlation was observed between the various cytokines examined. The functional effects observed were also diverse, with some supernatants showing strong inhibitory T cell activity, while others were stimulatory. Conclusion: Our results document the heterogeneity of the local cytokine microenvironment of premalignant cervical lessons, which may play a role in regulating the immune response associated with such lesions and hence influence clinical outcome.

CIN lesions
T cell proliferation
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