The Effects of Smoking during Pregnancy on Birth Outcomes in Southern Jordan

Background : Fetal exposure to maternal smoking has been implicated as a contributing factor to birth complications and subsequent developmental impairments in children. The aim of the present study was to investigate the association between maternal smoking and pregnancy outcomes in a sample of women giving birth at hospitals in southern Jordan. Methods : This observational study extracted data from the medical records of enrolled pregnant women, including demographic information, vital signs, and newborn measurements. Specific data included birth type (miscarriage or no miscarriage), birthweight, head circumference, Apgar score, and labor (term or pre-term). A two-tailed p -value of < 0.05 was considered statistically significant. Results : The study sample consisted of 410 pregnant women, comprising 114 smokers (smoking group) and 296 non-smokers (control group). Smokers were more likely to have lower parity, a lower gestational age upon labor, a lower birthweight ( < 2.5 kg), and they were less likely to have a pre-term labor compared to non-smokers. However, logistic regression could not determine any significant association with smoking. Smoking during pregnancy was not associated with an increased likelihood of miscarriage (odds ratio (OR): 1.22, 95% confidence interval (CI): 0.68–2.18, p = 0.50), low birthweight (OR = 0.70, 95% CI: 0.34, 1.45), or pre-term delivery (OR = 4.13, 95% CI: 2.27, 7.52). No significant associations were observed between smoking status and head circumference or Apgar score. Conclusions : Maternal smoking carries risks for pregnancy outcomes. Pregnant women who smoke are more likely to have low fetal birthweight and pre-term birth compared to non-smoking pregnant women. Our results highlight the need for comprehensive smoking cessation strategies targeted at pregnant women.


Introduction
Smoking during pregnancy has adverse health consequences for the mother and baby [1].It is associated with various complications including premature rupture of membranes, placenta previa, pre-term labor, spontaneous miscarriage, intrauterine growth retardation, fetal birth restriction, low birthweight and size, sudden infant death syndrome, as well as neurodevelopment disorders, cognitive disabilities, and long-term cancer risks in infants [2][3][4][5][6].Smoking can also lead to small head circumference, low Apgar score, stillbirths, and neonatal deaths.Moreover, smoking can increase the rate of cesarean births and the rates of morbidity and mortality in newborn infants [7].To reduce these risks, it is vital to minimize smoking by the mother throughout pregnancy and after birth.Several studies have reported differences in neonatal birthweight between mothers who are cigarette smokers and those who are non-smokers [8][9][10].
Passive, or involuntary smoking may be of particular importance in Jordan due to the high prevalence of passive smokers among women.Little is known about the dangerous effects on birth outcomes of smoking and even passive smoking throughout pregnancy [11].A self-report questionnaire study on exposure to second-hand smoke found that despite being aware of the importance of indoor environments on respiratory symptoms, family members in a significant proportion of households (71%) still smoked indoors [12].
The prevalence of tobacco smoking among pregnant women during pregnancy is estimated to be 9%, 12%, 16% and 7% in Germany, the UK, France, and the USA, respectively [13].It is difficult to accurately estimate the incidence of smoking by women during pregnancy, especially in socially disadvantaged areas.This is due to the social and medical pressures faced by pregnant women who smoke [14].In Jordan, the prevalence of smoking among women was reported to be 10.9%, with the highest inci-dence found in wealthier (15.5%) and less educated (17.2%) groups.Furthermore, urban areas had a higher incidence of female smokers (11.8%), and the highest prevalence was observed in 40-49 years old women (15.1%) [15,16].
The mechanisms underlying the harmful effects of smoking during pregnancy are not fully understood, but are likely to involve the nocive effects of nicotine and carbon monoxide.These reduce uteroplacental circulation, fetal tissue oxygenation and maternal weight gain, as well as having undesirable consequences for the fetus.In addition, the 4000 potentially toxic substances found in cigarettes may harm the health of the mother and baby.The effect of nicotine on brain development may also lead to behavioral problems in children [9].
The aim of the present study was to investigate the association between maternal smoking and pregnancy outcomes in a sample of women giving birth at hospitals in southern Jordan.We also examined associations between smoking and prematurity, Apgar score, birthweight, and head circumference.

Study Setting
This study was conducted from January to June 2022 at two major hospitals in Southern Jordan, namely the Al-Karak (411 beds) and Al-Tafila (244 beds) hospitals.

Study Population and Sampling
All women registered and admitted for labor at the delivery room in both hospitals during the study period were eligible for inclusion.The pregnant women were divided into two groups (smokers and non-smokers) at the time of delivery based on their response during evaluation.Currently active and passive smokers were classified as smokers.Smoking status was self-reported by the women during recording of their history.For the purpose of this study, the data on smoking was retrieved from medical records.Women were educated about the National Health Interview Survey (NHIS) definition of smoking.Those exposed to passive smoking were considered as smokers.Women were considered to be non-smokers when they did not meet the criteria of being a current smoker, a passive smoker, or an ex-smoker who quit either before or after becoming pregnant.Fig. 1 illustrates the inclusion process.This study was approved by the institutional review board (IRB) committee of our institute (No.1222023, Date 3.8.2023).Informed consent was waived due to the retrospective nature of the study.Patient data was anonymized to ensure the confidentiality of any identifiable information.This study conforms to the provisions of the Declaration of Helsinki.

Data Analysis
Following data entry, statistical analysis was conducted using the Statistical Package for the Social Sciences (SPSS) version 22 (IBM Corp., Armonk, NY, USA).Data were presented in tabular form (frequency and percentage) for the two study groups.Cross-tabulation and the Chisquare test were used to detect significant associations between the study variables and smoking, with Fisher's exact test used for low cell counts.Odds ratios (ORs) were calculated for variables showing a significant association with smoking.The 95% confidence interval (95% CI) was used to express uncertainty of results.A two-tailed p-value of <0.05 was considered statistically significant.

Results
Included in this study were a total of 410 women with a mean age of 29.7 (standard deviation: 6.1) years.Of these, 296 (72%) were classified as non-smokers and 114 as smokers.No significant difference in age was observed between smokers and non-smokers.Table 1 shows the demographic and clinical characteristics of the smoking and non-smoking groups.Parity was significantly different between the two groups, with 18% of non-smokers having parity ≥5 compared to only 4.4% of smokers (p < 0.001).The smoking group had a lower gestational age (35.0 weeks vs. 36.6weeks, p < 0.001), and a higher proportion of low birthweight deliveries (24% vs. 12%, p = 0.004) compared to the non-smoking group.Twelve women gave birth to infants with macrosomia.No significant differences between smokers and non-smokers were observed for the Apgar score.
The association between smoking and history of miscarriage was also examined using a logistic regression model.As shown in Table 2, this analysis showed no significant association between smokers and the risk of miscarriage (OR: 1.22, 95% CI: 0.68-2.18,p = 0.50).Age was significantly associated with a history of miscarriage (OR: 1.1, 95% CI: 1.0-1.12,p = 0.004), while higher birthweight (≥2.5 kg) was associated with no history of miscarriage (OR: 0.40, 95% CI: 0.19-0.81,p = 0.012).Term deliveries were also associated with no history of miscarriage (OR: 0.27, 95% CI: 0.10-0.75,p = 0.012).No significant associations were found between having a history of miscarriage and the gestational age, Apgar score, type of delivery, and head circumference.
The logistic regression model for the association between parity and study variables showed a significant asso-  ciation between lower Apgar score and patients with para 2 (OR: 1.89, p-value = 0.039).In addition, term deliveries were less likely associated with para 2 (OR: 0.24, p-value = 0.038).In women with para 3, there was a significant association with lower gestational age (OR: 0.75, p-value = 0.02), and vaginal deliveries were less likely seen in women with para 3 (OR: 0.39, p-value = 0.008), preterm deliveries were more likely to be associated with para 3 (OR: 0.23, p-value = 0.032).Lower gestational age was significantly associated with women with para 4 (OR: 0.76, p-value = 0.017), and term deliveries were less likely to be seen in para 4 (Table 3).
The logistic regression model for the association between birth weight and study variables showed a significant association between miscarriage and birth weight in which mothers delivering babies with ≥2.5 kg were more likely to not have miscarriage (OR: 2.5, p-value = 0.015).In addition, term delivers were associated with birth weight <2.5 kg (Table 4).
The logistic regression model (Table 5) investigating the association between labor and study variables showed a significant association between head circumference and labor in which ≥35 cm circumferences were associated with term deliveries (OR: 0.22, p-value = 0.009).In addition, birth weight ≥2.5 kg were associated with term deliveries (OR: 0.1, p-value < 0.001), and smoking mothers were more likely to have preterm labor (OR: 4.13, p-value < 0.001).

Discussion
This study estimated the prevalence of smokers and passive smokers amongst pregnant women in Southern Jordan.In addition, it investigated whether smoking in these women was associated with adverse pregnancy outcomes.Finally, logistic regression analysis was performed to evaluate the association between smoking and a history of miscarriage.
Although 26% of smoking women have had a history of miscarriage, we could not find a statistically significant association between smoking and miscarriage.These findings are in disagreement with those of Budani et al. [17], who reported a significantly higher rate of spontaneous miscarriage in women who were former smokers.Similarly, a systemic review conducted by Pineles et al. [18] found an increased rate of miscarriage in women who were active smokers, or who experienced second-hand smoking during pregnancy.George et al. [19] reported that pregnant women who were exposed to environmental tobacco smoke had a higher risk of spontaneous abortion than women who were not exposed (adjusted OR = 1.67).Furthermore, active smokers showed an elevated risk of spontaneous abortion compared to non-smokers.
The results of our study revealed that smoking during pregnancy was significantly associated with the risk of low birthweight.This finding agrees with those of other studies that reported associations between tobacco use during pregnancy and low birthweight, and with small fetus size during gestation [20,21].
The current study found no significant association between the head circumference of infants and smoking by the mother during pregnancy.This is inconsistent with the results of an earlier study that found smoking during pregnancy was associated with a smaller head circumference (mean of 0.27 cm) compared to non-smoking mothers (pooled weighted mean difference = 0.27; 95% CI: 0.25, 0.29) [22].This difference could be due to the small sample size in our study, and possibly also to ethnic differences.The present study found no significant association between the Apgar score and smoking during pregnancy.This may be due to the small sample size and the inability to calculate the score in a small number of newly born infants.The Apgar score is used to evaluate the health of newly born infants during the immediate neonatal period, with a score of <7 indicating a high risk of neonatal death [23].A previous study found the Apgar score at 5 minutes was lower in newborn infants from smoking mothers compared to nonsmoking mothers, although there was no detectable effect of quitting smoking during pregnancy [24].Garn et al. [25] reported that a low Apgar score was significantly correlated to the number of cigarettes smoked per day during pregnancy in both black and white mothers.Moreover, the first-minute Apgar score in newborn babies from smoking mothers was found to be significantly lower than in those from non-smoking mothers [26].
Our results showed a significant association between pre-term delivery and smoking during pregnancy, with a percentage of 49% (χ 2 = 34.8).Several studies have consistently reported an association between sustained smoking by mothers during pregnancy and an increased incidence of pre-term birth [27,28].Another study reported no association between pre-term birth and maternal smoking during the first trimester, with inconsistent results regarding the risk of low bodyweight.There is evidence for an independent effect of paternal smoking during pregnancy and pre-term birth, which may be explained by the harm- ful intrauterine effects of passive smoking [29].Therefore, strategies to reduce the consequences of smoking during pregnancy should also focus on paternal smoking before and at the beginning of pregnancy.
This study investigated a crucial association between smoking in pregnant women and birth outcomes.The results demonstrate the need for more awareness amongst pregnant women in Jordan regarding the negative effects of smoking.Limitations of our study include the small sample size of both the case and control groups, the retrospective nature of data collection, the lack of data regarding body mass index, the inaccurate definition of smoking, the failure to use specific or non-specific smoking tests to validate the smoking and non-smoking groups, and the failure to consider other smoking alternatives such as the water pipe, ecigarettes and vaping.
Future research should explore the mechanisms underlying the adverse effects of smoking on pregnancy outcomes.The impact of smoking cessation at different stages of pregnancy on birth outcomes should also be investigated with the aim of identifying critical windows for intervention.In addition, future studies should have larger sample sizes and a prospective design in order to minimize recall bias and improve the accuracy of classification for smoking.Possible effects of alternative smoking methods (e.g., vaping, water pipe) on pregnancy outcomes should also be investigated, given their increasing popularity.Finally, research into the role of paternal and household smoking will provide a better understanding of the impact of tobacco exposure on pregnancy and neonatal health.

Conclusions
In conclusion, the results of the present study revealed smoking during pregnancy might be related to birth outcomes including low birthweight, pre-term birth, miscarriage, head circumference and the Apgar score.However, we could not find any statistical significance when testing for direct association.Future studies should examine the effects of maternal smoking on the fetus in utero, the effects of maternal smoking during the three different trimesters of pregnancy, the effects of smoking cessation before and during the different trimesters of pregnancy, and the effects of passive and paternal smoking.