Objectives: This study aimed to compare the feasibility and advantages of a three-dimensional rotational angiography (3DRA) road map for individualized guidance of uterine artery (UA) catheterization with those of a conventional two-dimensional (2D) road map. Materials and Methods: Twenty patients were randomly assigned to the 3DRA and 2D groups for UA catheterization and embolization for symptomatic uterine fibroid, postpartum haemorrhage, incision pregnancy, adenomyosis, and cervical cancer in the past 2 years at our hospital. Demographic data such as patient age, abdominal perimeter, and uterine diseases were recorded. Procedure time, fluoroscopy time, contrast medium volume, and patient radiation dose were also recorded. There was no difference between the two groups in terms of demographic data. Results: All 20 patients were successfully catheterized with the guidance of the 3DRA or 2D road map. Patients with the 3DRA road map had shorter total procedure time, catheterization time, fluoroscopy time, and smaller contrast medium volume and irradiation. Conclusions: the 3DRA road map is feasible and useful for the individualized guidance of UA catheterization and preferable to the conventional 2D road map.