IMR Press / CEOG / Volume 46 / Issue 2 / DOI: 10.12891/ceog4513.2019

Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 47 Issue 1 (2020). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
Downregulation of Hsp27 inhibits proliferation, migration and invasion in human choriocarcinoma cell line JAR
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1 Shaoxing Women and Children's Hospital, Shaoxing, China
*Correspondence: xiaoliangshi0822@163.com (XIAO-LIANG SHI)
Contributed equally.
Clin. Exp. Obstet. Gynecol. 2019, 46(2), 245–249; https://doi.org/10.12891/ceog4513.2019
Published: 10 April 2019
Abstract

Purpose of Investigation: Heat shock protein 27 (Hsp27), a member of the heat shock protein (Hsp) family, is critical in the stress response. However, the role of Hsp27 in the pathogenesis of preeclampsia remains unknown. The aim of the present study was to examine effect of downregulation of Hsp27 in proliferation, migration, and invasion in human JAR cells. Materials and Methods: To determine their effect, siRNA interference was used to knock down Hsp27 expression in JAR lines. The effects of transfected cells were screened for HSP27 expression by Western blotting analysis. Cell proliferation determined by CCK-8 assay and cell clonogenic assay. Wound healing assay measured the migration ability. Transwell assay measured migration and invasion ability. Results: Downregulation of Hsp27 inhibits proliferation, migration, and invasion in human choriocarcinoma cell line JAR. Conclusion: The present data suggested that Hsp27 may play an important role in preeclampsia.

Keywords
Hsp27
siRNA
Proliferation
Migration
Invasion
Figures
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