IMR Press / CEOG / Volume 45 / Issue 6 / DOI: 10.12891/ceog4355.2018

Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 46 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
Decreased plasma concentrations of pregnancy-associated placenta-specific microRNAs in pregnancies with a diagnosis of fetal trisomy 18
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1 Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Clin. Exp. Obstet. Gynecol. 2018, 45(6), 897–900; https://doi.org/10.12891/ceog4355.2018
Published: 10 December 2018
Abstract

Objectives: This study aimed to clarify the association between circulating pregnancy-associated, placenta-specific microRNAs in the maternal plasma of women with fetal trisomy 18 pregnancies. Materials and Methods: All samples were obtained after receiving written informed consent and the study protocol was approved by the Institutional Review Board. Maternal blood samples (7 ml) were obtained at 16–19 weeks of gestation from 13 pregnant women carrying fetuses with trisomy 18 (trisomy 18 pregnancy group) and from 24 pregnant women carrying fetuses with normal karyotypes (normal karyotype pregnancy group). The plasma concentrations of pregnancy- associated, placenta-specific microRNAs (miR-515-3p, -517a, -517c, and -518b) were measured by quantitative RT-PCR. Results: No significant differences in clinical characteristics were observed between the two groups. miR-515-3p, miR-517a, miR-517c, and miR- 518b were shown to be present in significantly lower plasma concentrations in the trisomy 18 pregnancy group than in the normal karyotype pregnancy group. Plasma concentrations of cell-free miR-517c could distinguish pregnancies with fetal trisomy 18 from those with normal fetal karyotypes, yielding an area under the curve of 0.733 (95% confidence interval: 0.559–0.881). Conclusion: Cell-free pregnancy- associated, placenta-specific microRNAs may therefore be potential molecular markers to estimate the risk of pregnancies with fetal trisomy 18.
Keywords
Placenta-specific microRNA
Pregnancy
Fetal trisomy 18
Maternal plasma
Molecular marker
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