IMR Press / CEOG / Volume 45 / Issue 6 / DOI: 10.12891/ceog4351.2018

Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 46 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
Decreased cell-free but not exosomal miR-518b in maternal plasma is caused by amniocentesis
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1 Department of Obstetrics and Gynecology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
2 Department of Human Genetics, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan
Clin. Exp. Obstet. Gynecol. 2018, 45(6), 875–879; https://doi.org/10.12891/ceog4351.2018
Published: 10 December 2018
Abstract

Aim: To obtain knowledge of the relationship between cell-free and exosomal pregnancy-associated microRNAs in maternal plasma, the authors investigated their circulating levels before and after amniocentesis. Materials and Methods: In 16 maternal plasma samples collected before and after amniocentesis, circulating levels of cell-free and exosomal pregnancy-associated microRNAs (miR-515-3p, -517a, -517c, -518b, and 323-3p) were measured by real-time quantitative RT-PCR. Changes in the plasma concentration and the association between circulating levels of cell-free and exosomal microRNAs were analyzed. Statistical significance was defined as p < 0.05. Results: The plasma concentration of cell-free miR-518b was significantly decreased after amniocentesis (Wilcoxon signed rank test, p = 0.001), but no significant difference was observed in any other cell-free or exosomal microRNA (p > 0.05). There was no association between the circulating levels of cell-free and exosomal microRNAs (miR-515-3p, -517a, -517c, -518b) in chromosome 19 microRNA cluster region. However, a significant association was detected between plasma cell-free and exosomal miR-323-3p levels in chromosome 14 microRNA cluster region (r and P values before amniocentesis; 0.617 and 0.011, those after amniocentesis; 0.899 and < 0.001). Conclusions: Amniocentesis caused a decreased cell-free but not exosomal miR-518b levels in maternal plasma. Correlations between plasma concentrations of cell-free and exosomal pregnancy-associated microRNAs were different for each microRNA.
Keywords
MicroRNA
Amniocentesis
Cell-free
Exosome
Maternal plasma
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