IMR Press / CEOG / Volume 45 / Issue 5 / DOI: 10.12891/ceog4279.2018

Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 46 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
Disturbed angiogenesis in intrauterine growth restriction-compromised placentas at term
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1 Department of Gynaecology and Obstetrics, First Faculty of Medicine and Hospital Bulovka, Charles University, Prague, Czech Republic
2 Institute of Inherited Metabolic Disorders, First Faculty of Medicine, Charles University and General University Hospital in Prague, Prague, Czech Republic
3 Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic
Clin. Exp. Obstet. Gynecol. 2018, 45(5), 707–712; https://doi.org/10.12891/ceog4279.2018
Published: 10 October 2018
Abstract

Purpose of investigation: The aim of this study was to compare levels of selected angiogenesis-related proteins in intrauterine growth restriction (IUGR)-compromised and normal placentas at term. Materials and Methods: Stromatal tissue of placental villi of gestationally matched IUGR patients and controls (both groups of n=32, gestational age between 39+0 and 40+6 gtt) was separated by microdissection. After separation, placental tissue was homogenised on ice (2′10 min, 2,000 rpm) and centrifuged (2'5 min, 5,000 rpm). Proteins of supernatant were fractionated by 8% or 10% SDS-PAGE and Tie-1, Tie-2, VEGFR-1, VEGFR-2, and MMP-2 levels were measured by Western blot using appropriate primary and secondary antibodies. Results: In the IUGR group, compared to the control group, there was a significantly lower level of Tie-1 (67.1% of controls, p < 0.001), VEGFR-1 (72.4% of controls, p < 0.001), VEGFR- 2 (68.5% of controls, p = 0.001), and a significantly higher level of Tie-2 (119% of controls, p = 0.023). MMP-2 was increased in the IUGR group (127% of controls, p <0.001). Conclusions: Placental levels of Tie-1, Tie-2, VEGFR-1, and VEGFR-2 are altered in pregnancies complicated by IUGR. Compared with that in normal pregnancies, the level of MMP-2 was upregulated. The present authors speculate that the results of this study represent the angiogenic imbalance observed in IUGR pregnancies; thereby resulting in poor and disturbed angiogenesis underlying delayed development of the fetus.
Keywords
IUGR
Placenta
Angiogenesis
Angiogenic factors
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