Purpose of investigation: To compare surgical outcomes of laparoendoscopic single-site myomectomy (LESS-M) and three-port conventional laparoscopic myomectomy (CLM). Materials and Methods: Medical records and videos of 158 patients (79 LESS-M and 79 CLM) were reviewed retrospectively. The authors analyzed technical feasibility and operative outcomes for all patients. Results: Patient characteristics were similar in the two groups. There were no significant differences in the number of myomas (2.24 [1–8] vs. 2.41 [1–12]; p = 0.571) and size of the largest myoma (7.04 [4–15] vs. 6.89 [3–15] cm; p = 0.689) between the groups. LESS-M resulted in a significant decrease in blood loss (189 vs. 273 mL; p = 0.020). Postoperative pain scores were significantly lower in the LESS-M group at one and six hours after surgery. Conclusions: LESS-M using V-Loc and extracorporeal suture technique with Polysorb is associated with less blood loss and less postoperative pain.