IMR Press / CEOG / Volume 45 / Issue 1 / DOI: 10.12891/ceog3825.2018

Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 46 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
The expression of sirtuin1 in normal and preeclamptic villous explants under hypoxia
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1 Department of Obstetrics and Gynecology, Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, Korea
2 Institute of Women's Life Medical Science, Yonsei University College of Medicine, Seoul, Korea
Clin. Exp. Obstet. Gynecol. 2018, 45(1), 75–80; https://doi.org/10.12891/ceog3825.2018
Published: 10 February 2018
Abstract

Purpose of investigation: To investigate the expression of sirtuin1 and hypoxia-inducible factor-1α in normal and preeclamptic placenta under hypoxia. Materials and Methods: Twelve women with severe preeclampsia and ten normotensive women were enrolled in the study. Villous explants from the placenta were collected following cesarean delivery. Quantitative reverse transcription polymerase chain reaction, Western blot analysis, and immunohistochemical staining were performed to evaluate mRNA expression, as well as to quantify and identify the tissue localization of SIRT1 and HIF-1α in each placenta. Results: SIRT1 mRNA expression was lower in preeclamptic villous explants than in normal explants, while HIF-1α mRNA expression was higher in the preeclamptic placenta than in the normal placenta. Expression of SIRT1 mRNA decreased under hypoxia, although expression of HIF-1α mRNA increased after 6 and 24 hours of hypoxia. Conclusion: Down-regulation of SIRT1 might be associated with stabilization of HIF-1α following prolonged hypoxia in preeclamptic placenta.
Keywords
Sirtuin1
Hypoxia-inducible factors-1
alpha Subunit
Pre-Eclampsia
Pl
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