Aims: To investigate the effect of cervical conization on the outcome of in-vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) treatment. Materials and Methods: The authors performed a retrospective, database-searched cohort study based on patients undergoing controlled ovarian hyperstimulation and IVF/ICSI between 2009 and 2013 in the present hospital. Cervical intraepithelial neoplasia (CIN) or cervical cancer was carefully confirmed by transvaginal ultrasound, hysteroscopy, and biopsy. High-quality case-control study with strict inclusion criteria was conducted. The authors analyzed basic characters and main IVF/ICSI outcomes between both groups. Results: The authors included 48 patients with a history of cervical conization who underwent IVF/ICSI and control group without cervical conization. No significant differences were found in IVF/ICSI outcomes between both groups. No obvious evidence was found indicating that cervical stenosis could impact IVF operation. Conclusions: The present results suggest that cervical conization does not affect IVF/ICSI outcomes. Patients can receive cervical conization before undertaking assisted reproductive technology.