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Original Research
Effects of apelin on proliferation and apoptosis in rat ovarian granulosa cells
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1
Department of Gynecology and Obstetrics, Suining Central Hospital, Suining
2
Department of Gynecology and Obsterics, Jinan Central Hospital Affiliated Shandong University, Jinan
3
Deparment of Gynecology and Obsterics, Zhongshan Hospital, Wuhan
4
Department of Gynecology and Obstetrics, the First Affiliated Hospital of Chongqing Medical University, Chongqing (China)
Clin. Exp. Obstet. Gynecol. 2016, 43(3), 409–413;
https://doi.org/10.12891/ceog2133.2016
Published: 10 June 2016
Abstract
Objective: To investigate the influence of apelin on proliferation and apoptosis in rat ovarian granulosa cells (GCs) in vitro. Materials and Methods: Primary culture of SD rat ovarian GCs was cultured with different concentrations of apelin 10-8 mol/L, and APJ expression in GCs was inhibited by small RNA interfering (siRNA). Signaling inhibitor LY294002 of PI3K/Akt, HIMO intervention, MTT assay and flow cytometry were combined to observe the cell proliferation and apoptosis; Western blot was used to detect signaling protein expression related with cell apoptosis. Results: Compared with the control group, the differences on cell proliferation rate detected by MTT assay in APJ-siRNA group, LY294002 group, and HIMO group had statistical significance (p < 0.05). Protein expressions of Bad, Bax, and Foxo3a in GCs in APJ-siRNA group, LY294002 group, and HIMO group were apparently upregulated (p < 0.05), and the protein expression of Bcl-2 in APJ-siRNA group, LY294002 group, and HIMO group was obviously downregulated (p < 0.05). Conclusion: Apelin can promote proliferation of GCs and inhibit apoptosis via PI3/Akt signaling pathway.
Keywords
Apelin
Granulosa cells
PI3K/Akt