IMR Press / CEOG / Volume 43 / Issue 1 / DOI: 10.12891/ceog2035.2016

Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 46 Issue 1 (2019). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Open Access Original Research
The effects of melatonin on endometriotic lesions induced by implanting human endometriotic cells in the first SCID-mouse endometriosis-model developed in Turkey
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1 Department of Obstetrics and Gynecology, Yeditepe University, Istanbul
2 Department of Pathology, Yeditepe University, Istanbul
3 Experimental Research Center of Yeditepe University (YUDETAM), Istanbul (Turkey)
Clin. Exp. Obstet. Gynecol. 2016, 43(1), 25–30; https://doi.org/10.12891/ceog2035.2016
Published: 10 February 2016
Abstract

Objective: To evaluate the effects of melatonin on endometriotic lesions induced by implanting human endometriotic cells in SCID mice. Materials and Methods: Prospective, randomized, controlled, experimental study. Experimental Research Center of Yeditepe University (YUDETAM). Thirty female, non-pregnant, nulligravid severe combined immunodeficient (SCID) mice. Endometriotic cells collected from patients with endometriosis were implanted subcutaneously in 30 SCID mice. These mice were randomized into two study groups: in the first group, mice were administered melatonin (20 mg/kg/day) following induction of endometriosis for four weeks; in the second group, nothing was administered. All the mice were given a high dose of exogenous estradiol (50 μg/kg/d, twice weekly). Four weeks after inoculation, necropsies were performed and endometriotic lesions were collected. All the lesions were evaluated histopathologically and the levels of SOD and MDA were assessed in the lesions. Results: Successful implantation was observed in the 28 mice that survived. Mean MDA level was 5.0±1.7 and 8.8±2.6 in the melatonin and control groups, respectively (p = 0.01); mean SOD level was 1.1 ± 0.1 and 1.0 ± 0.1 in the melatonin and control groups, respectively (p = 0.49). Mean histopathological score was lower in the melatonin group (p = 0.04). Conclusions: Melatonin was effective in the treatment of experimental endometriosis induced in SCID mice.
Keywords
Benning ovarian cystic teratoma
2D/3D/4D HDLive
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