Objective: To study the relevance of PTH, 25(OH)D3, CT, bone resorption markers C-terminal telopeptide of type I (CTX-1), andtartrate-resistant acid phosphatase (TRACP), bone formation markers bone gla protein (BGP), bone alkaline phosphatase (BALP) withthe femoral neck BMD in females. Materials and Methods: PTH, 25(OH)D3, CT, CTX-1, TRACP, BGP, and BALP were detected byan enzyme immunoassay analyzer and femoral neck BMD were measured by a BMD detector. The results of 860 females were dividedinto several groups according to standard of five-year age intervals. SPSS 13.0 software was used for statistical analysis. Results: Themeasured values of PTH, 25(OH) D3 and CT had no differences in 35-50 age group. The measured values of 25(OH) D3 began to declineafter the age of 50, and 25(OH)D3 had positive relevance with BMD. The values of CT were decreased in the age groups from 65to 79 years old, and were significant positive correlated with BMD. The CTX-1 and TRACP had negative relevance with BMD in 35-45 age group and BGP and BALP had positive relevance with BMD in 35-45 age group. The BGP, BALP increased significantly in 50-60 age group, and CTX-1, TRACP, BGP, and BALP had negative relevance with BMD in 50-60 age group. BGP and BALP began todecline and had positive relevance with BMD after the age of 65, and CTX-1 and TRACP had negative relevance with BMD after theage of 65. Conclusions: PTH, 25(OH)D3, CT, CTX-1, TRACP, BGP, and BALP were the important technical means for monitoring thelevel of bone metabolism and the diagnosis and differential diagnosis of osteoporosis.