Objective: To analyze the clinical effects of laparoscopic sacral colpopexy using prolene mesh for patients with pelvic organ prolapse. Methods: Laparoscopic placement of prolene mesh in the rectovaginal septum and veslcovaginal septum of 42 women with uterine prolapse with bladder and rectum prolapse. One mesh was fixed to the round ligaments and another to the periosteum of the sacral vertebrae. Operation time, blood loss, complication rate and follow-up surgery results were recorded monthly and analyzed according to the POP-Q system. The uterus was preserved in all cases. Results: The mean operating time was 92 ± 12 min and the mean blood loss during surgery was 98 ± 11 ml. Postoperatively, both prolapse and symptoms were highly significantly improved (p < 0.001) according to the pelvic organ prolapse quantification (POP-Q) system. Conclusions: Laparoscopic sacral colpopexy for uterine prolapse using prolene mesh is a minimally invasive and effective new technique that offers a chance for patients who desire to preserve their uterus.