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Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 47 Issue 1 (2020). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.
Original Research
Cryopreservation of blastocysts using a modification of a simplified freezing protocol with a one step removal of cryoprotectant successfully used previously to freeze 2 pronuclear or multi-cell embryos
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The University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School at Camden, Cooper Hospital/University Medical Center, Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology & Infertility, Camden, NJ (USA)
Clin. Exp. Obstet. Gynecol. 2010, 37(2), 99–99;
Published: 10 June 2010
Abstract
Purpose: To describe a modification of a simplified freezing protocol for the cryopreservation of blastocysts. Methods: 1.5 M glycerol was substituted as a cryoprotectant instead of propanediol. Results: There was a survival rate of 59.1% (13/22) with three live deliveries in seven transfers (42.9% per transfer). The implantation rate was 28.6% (4/14). Conclusions: This is the first description of a new technique for freezing blastocysts. A larger series is needed to determine if the good pregnancy rates will continue.
Keywords
Blastocyst
Cryopreservation
Implantation rates