Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 47 Issue 1 (2020). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.
A comparison of in vitro fertilization outcome by culture media used for developing cleavage-stage embryos
Purpose: To determine whether removing glucose and phosphate from media used for developing cleavage-stage embryos improves outcome following transfer of fresh or frozen embryos. Furthermore the study would evaluate the efficacy of adding nonessential amino acids and glutamate to media. Methods: Embryo development was rotated on a weekly basis in human tubal fluid (HTF), versus two media relatively ·devoid of glucose and phosphate (e.g., Pl), with one having the addition of essential amino acids and glutamate (Quinn’s Advantage Medium). Results: For fresh cycles, the implantation rate was significantly higher for Quinn's. There was less fragmentation with Pl and Quinn's. For frozen cycles, the viable pregnancy, implantation rates and embryo quality were higher for Quinn's and Pl than HTF. Conclusion: Removal of glucose and phosphate for day-2 embryos improves in vitro fertilization outcome after embryo transfer. It is not clear if adding certain non-essential amino acids and glutamate provides further improvement.