Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 47 Issue 1 (2020). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.
Cryoloop vitrification in assisted reproduction: analysis of survival rates in > 1000 human oocytes after ultra-rapid cooling with polymer augmented cryoprotectants
While human oocytes have been successfully limited the routine been clinical application of oocyte as an .alternative laboratory approach to long-term oocyte preservation in assisted reproduction, .there is little agreement on how best to configure such cryopreservation protocols to optimize oocyte. To compare post-thaw oocyte survival rates, we performed cryoloop vitrification of human oocytes utilizing two different cryoprotectant mixtures that included polymer macromolecules. Human oocytes (n = 1120) were obtained from consenting patients undergoing in vitro fertilization, but only failedmatured or failed-fertilized (inseminated but without 2pn development) were included in this investigation. Protocol A consisted of 20% ethylene glycol and 20% dimethyl sulphoxide + 0.4 M sucrose and 20% synthetic serum substitute. Protocol B consisted of 20% ethylene glycol and 20% dimethyl sulphoxide + 0.65 M sucrose, 1 mg/mL polyethylene glycol, IO mg/mL Ficoll and 20% synthetic serum substitute. Following cryostorage for 10-14 d at -l96°C, the survival rate for oocytes vitrified with protocol A was 80.9%，whereas the post-thaw among protocol Boocytes was 80.6% (p > 0.005). results indicate that an ethylene glycol+ dimethyl sulphoxide mixture (with or without polymer macromolecules) can be an effective cryoprotectant strategy for human oocyte vitrification; either approach can be employed without any observed compromise in post-warming survival and/or morphology.