IMR Press / CEOG / Volume 28 / Issue 3 / pii/2001044

Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 47 Issue 1 (2020). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on imrpress.com as a courtesy and upon agreement with S.O.G.

Original Research

Cryopreservation of oocytes after vitrification

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1 Faculty of Medicine and Surgery, Institute of Gynecology and Obstetrics, University of Messina (Italy)
2 Faculty of Veterinary Medicine, Institute of Gynecology and Obstetrics, Division of Physiopathology of Reproductwn, University of Milan (Italy)
Clin. Exp. Obstet. Gynecol. 2001, 28(3), 157–162;
Published: 10 September 2001
Abstract

Recently the majority of studies and research in the field of physiopathology of reproduction have been oriented towards cryo­preservation of female eggs. This could resolve the ethical problems connected with the preservation of the embryo and with the gestation of the surplus oocytes collected after cycles of ovarian hyperstimulation.

Notwithstanding that extraordinary results have been obtained in the field of cryopreservation of embryos and spermatozoa, the freezing of oocytes involves unfortunately many difficulties in relation, above all, to deep morphostructural and functional modifi­cations which take place due to the low temperatures and to the toxic action of cryoprotective agents.

The aim of this work was to test and then verify whether an efficient method of preserving the female egg cells by freezing m order to be able to subsequently use them for in vitro fertilization.

The conclusions of this study, even if not highly encouraging, have allowed us to better evaluate the damage caused by low tem­peratures and at the same time should stimulate us and other researchers in this field to find new methods which will allow us to reach higher aspirations.

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