IMR Press / CEOG / Volume 20 / Issue 3 / pii/1993023

Clinical and Experimental Obstetrics & Gynecology (CEOG) is published by IMR Press from Volume 47 Issue 1 (2020). Previous articles were published by another publisher on a subscription basis, and they are hosted by IMR Press on as a courtesy and upon agreement with S.O.G.

Original Research

Functional analysis of tumor - associated lymphocytes from gynecological tumors

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1 Center of Biological Chemistry and Division of Gynecologic Oncology, Dreieich, (Germany)
2 Department of Gynecology and Obstetrics, Division of Gynecologic Oncology, Dreieich, (Germany)
3 University Medical Center Frankfurt am Main and Biotest Pharma GmbH, Dreieich, (Germany)
Clin. Exp. Obstet. Gynecol. 1993, 20(3), 137–144;
Published: 10 September 1993

Tumor-Associated Lymphocytes (TAL) were isolated from peritoneal fluids of six ovarian cancer patients and pleural effusion from eight breast cancer patients, respectively. In one case we obtained ascitic fluid as well as pleural effusion because of intraabdominal metastatic breast carcinoma. The collected cells were cultured in a complete medium and supplemented with human interleukin-2 (nIL-2) in a concentration of 1000 Units/ml. Phenotyping was not always possible due to rapid decay of the cells. Cytotoxicity was determined with a fluorescence-based assay, in some cases at different stages of cell growth. In two cases TAL from ascitic fluids showed increased cytotoxic activity after a longer cultivation period. TAL from pleural effusions showed cytotoxic activity against the target cell lines in two cases only. Some of these TAL did not proliferate any more but died within 24 h. With the functional analysis we wanted to investigate the cytotoxic potential against natural killer (NK)-sensitive and NK-resistant (Raji) cell lines. The results demonstrate the ability of some of the TAL populations to destroy tumor cells.

Tumor-associated lymphocytes
Cytotoxic activity
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